Figure 1. A schematic view of the whole procedure.

Rag2^-/- mice were first treated with rIL-33_95-270 to expand ILC2s in vivo (see Procedure A). Lin^-CD45⁺ ILC2s are then isolated (see Procedure B) and cultured for several days (see Procedure C), before being used for subsequent ex vivo or in vivo experiments.