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. 2018 Oct 5;8(19):e3032. doi: 10.21769/BioProtoc.3032

Figure 2. Density gradient separation procedure.

Figure 2.

A. Before centrifugation, lymphoid cells are located in the cell suspension phase above the Lympholyte®-M. B. After centrifugation, lymphoid cells are concentrated in the interface between the DPBS buffer and the Lympholyte®-M. Red blood cells, dead cells and debris are found at the bottom of the tube.