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. 2018 Oct 20;8(20):e3046. doi: 10.21769/BioProtoc.3046

Figure 6. Super-resolution imaging of adult brain slices (Thy1-YFP-H mouse).

Figure 6.

Due to the minimized spherical aberrations, SeeDB2S is also useful for various kinds of super-resolution microscopy, such as STED (Leica), Airyscan (Zeiss), HyVolution2 (Leica), and SpinSR (Olympus). For large-scale super-resolution imaging, we first imaged with a 20x objective lens. Then, we focused on a particular neuron and obtained super-resolution images along the target neuron to save imaging time and data volume. We used Neurolucida (MBF Bioscience) for the quantification and reconstruction of neuronal morphology. Images adapted from Ke et al. (2016) .