Video 1. Imaging chamber preparation and sample mounting.

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Cut a silicone rubber sheet (1 mm-thick and 0.2 mm-thick are shown in this video) into an appropriate size with scissors or a utility knife. A silicone rubber sheet will adhere to a glass slide, by removing air between the rubber sheet and the glass slide. Mount cleared samples (cleared samples are often difficult to find) using a perforated spoon and a paint brush. Use No. 1.5H coverslips to seal the samples.