Solutions	Composition
SeeDB2G with saponin (clearing)	2% saponin in Omnipaque350
SeeDB2G (mounting)	Use Omnipaque350 directly Or 7,500 μl Tris-EDTA (pH 7.6) + 10 g Histodenz
SeeDB2S with saponin (clearing)	3,780 μl Tris-EDTA (pH 7.6) + 420 μl 20% saponin solution + 10 g Histodenz
SeeDB2S (mounting)	4,200 μl Tris-EDTA (pH 7.6) + 10 g Histodenz Add 4,200 μl Tris-EDTA to a 50 ml conical tube, and then add 10 g Histodenz. Use a rotator to fully dissolve the Histodenz in the conical tube