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| Actin mix | Stock Concentration | Final Concentration | Volume (200 μl) |
|---|---|---|---|
| Globular Actin (G-Actin) | 30 μM* | 9.5 μM | 63.3 μl |
| Pyrene-labeled Actin | 15 μM * | 0.5 μM | 6.67 μl |
| EGTA | 5 mM | 200 μM | 8 μl |
| MgCl2 | 2.5 mM | 109.7 μM | 8.78 μl |
| G-Buffer | 113.22 μl** (Top up to 200 μl) |
