Table 2.
Sample name | Total reads | Total reads (bp) | GC% | Error rate (%) | Q20 (%) | Q30 (%) |
---|---|---|---|---|---|---|
cont1 | 67572136 | 10135820400 | 48.61 | 0.11 | 98.64 | 96.29 |
cont2 | 66474707 | 9971206050 | 47.63 | 0.11 | 98.75 | 96.62 |
infect1 | 69033293 | 9854993950 | 49.75 | 0.13 | 98.26 | 95.53 |
infect2 | 70292999 | 10543949850 | 49.98 | 0.13 | 98.33 | 95.57 |
Total reads: statistics of total reads, each adjacent four lines contains the information of one read, and the total read number of each file is calculated; total base: the number of all bases in total data; clean reads: filter the original data, remove the linker sequence, remove the contaminated part, and remove the sequence containing more low-quality bases to obtain clean reads; GC%: the percentage of G and C in all bases; error rate: the error rate of sequencing; Q20, Q30: the percentage of total number of bases where the Phred score is greater than 20 and 30 which indicates base call accuracy.