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. 2021 Jul 29;2021:8460355. doi: 10.1155/2021/8460355

Table 2.

Statistics of raw data quality.

Sample name Total reads Total reads (bp) GC% Error rate (%) Q20 (%) Q30 (%)
cont1 67572136 10135820400 48.61 0.11 98.64 96.29
cont2 66474707 9971206050 47.63 0.11 98.75 96.62
infect1 69033293 9854993950 49.75 0.13 98.26 95.53
infect2 70292999 10543949850 49.98 0.13 98.33 95.57

Total reads: statistics of total reads, each adjacent four lines contains the information of one read, and the total read number of each file is calculated; total base: the number of all bases in total data; clean reads: filter the original data, remove the linker sequence, remove the contaminated part, and remove the sequence containing more low-quality bases to obtain clean reads; GC%: the percentage of G and C in all bases; error rate: the error rate of sequencing; Q20, Q30: the percentage of total number of bases where the Phred score is greater than 20 and 30 which indicates base call accuracy.