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. 2021 May 4;9(3):871–882. doi: 10.1002/iid3.442

Figure 3.

Figure 3

Effects of TG2 on serum allergen‐specific antibodies, cytokines, and mRNA expression in mouse lung tissue. Serum OVA‐specific IgG1, IgG1/IgG2a ratio, IgE, and IL‐5 in BAL fluid after OVA challenge (A). IL‐4 (upper panel) or IL‐13 (lower panel)‐produced CD4+ in CD3+ cells were evaluated from pooled lung per group (n = 5–6 mice per group) using flow‐cytometry (B). % indicates the population in CD3+ cells. Real‐time PCR was performed to monitor the changes in mRNA levels in lung tissue. The expression of IFN‐γ, MUC5AC, TGF‐β, and Col4a1 mRNA evaluated after the OVA challenge (C). The levels of mRNA are expressed as a ratio with β‐actin. PLA2 activity and CysLT level were measured in BAL fluid (D). BAL, bronchoalveolar lavage; IL‐4, interleukin‐4; OVA, ovalbumin; CysLT, cysteinyl leukotriene; TG2, transglutaminase 2. *p < .05, **p < .01.