Figure 3.

PD suppressed the inflammatory response in LPS‐induced macrophages. (A) Cell counting kit‐8 (CCK‐8) analyzed the effects of PD (100, 200, 300, and 400 μM) on the viability of RAW264.7 cells (the number of repetition is 5, n = 5). (B–E) RAW264.7 cells were treated using PD for 1 h followed by treatment with LPS (1 μg/ml) for 6 h. The level of (B) TNF‐α, (C) IL‐6, (D) IL‐4, (E) IL‐10 protein was determined by ELISA (n = 3). (F–H) RAW246.7 cells were treated using PD for 1 h followed by stimulating using LPS for 12 h, the protein was used to analyses the expression of iNOS and COX‐2 by Western blot (n = 3). COX‐2, cyclooxygenase‐2; ELISA, enzyme‐linked immunosorbent assay; IL, interleukin; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; PD, polydatin; TNF, tumor necrosis factor. *p < 0.05, **p < 0.01, and ***p < 0.001