Figure 4.

PD maintained intestinal epithelial barrier integrity by inhibiting the secretion of inflammatory cytokines. RAW264.7 cells were stimulate using LPS (1 μg/ml) for 12 h, next, the culture medium was collected and centrifuged, and used to treat the MCEC cells for 12 h. RT‐PCR was performed to analyses the level of (A) Claudin‐1, (B) Occludin, and (C) ZO‐1 mRNA in MCEC cells (N = 3). LPS, lipopolysaccharide; mRNA, messenger RNA; PD, polydatin; RT‐PCR, reverse transcription polymerase chain reaction. *p < 0.05, **p < 0.01, and ***p < 0.001