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. 2021 May 19;9(3):959–970. doi: 10.1002/iid3.455

Table 1.

The sequence of primers used for RT‐PCR

Gene Primer sequence
TNF‐α 5′‐CCCCAAAGGGATGAGAAGTTC‐3′ 5′‐CCTCCACTTGGTGGTTTGCT‐3′
IL‐6 5′‐CCAGAAACCGCTATGAAGTTCC‐3′ 5′‐GTTGGGAGTGGTATCCTCTGTGA‐3′
IL‐1β 5′‐GTTCCCATTAGACAACTGCACTACAG‐3′ 5′‐GTCGTTGCTTGGTTCTCCTTGTA‐3′
iNOS 5′‐GAACTGTAGCACAGCACAGGAAAT‐3′ 5′‐CGTACCGGATGAGCTGTGAAT‐3′
COX‐2 5′‐CAGTTTATGTTGTCTGTCCAGAGTTTC‐3′ 5′‐CCAGCACTTCACCCATCAGTT‐3′
β‐actin 5′‐GTCAGGTCATCACTATCGGCAAT‐3′ 5′‐AGAGGTCTTTACGGATGTCAACGT‐3′
ZO‐1 5′‐GACCTTGATTTGCATGACGA‐3′ 5′‐AGGACCGTGTAATGGCAGAC‐3′
Claudin‐1 5′‐AGGTCTGGCGACATTAGTGG‐3′ 5′‐CGTGGTGTTGGGTAAGAGGT‐3′
occludin 5′‐ACACTTGCTTGGGACAGAGG‐3′ 5′‐AAGGAAGCGATGAAGCAGAA‐3′
β‐actin 5′‐GTCAGGTCATCACTATCGGCAAT‐3′ 5′‐AGAGGTCTTTACGGATGTCAACGT‐3′

Abbreviation: RT‐PCR, reverse‐transcription polymerase chain reaction.