Figure 3.

Lipo α‐GC increases donor iNKT cells, decreases host iNKT cells, and expands donor Tregs in an early phase after BMT. Tregs were defined as CD4⁺FoxP3⁺CD25⁺ cells and iNKT cells were defined as CD19⁻TCR‐β⁺CD1d tetramer⁺ cells. Chimerism was evaluated in the host (Ly9.1⁺) or donor (Ly9.1⁻). (A) The number of donor iNKT cells and host iNKT cells in spleen cells after BMT in vehicle (n = 5, one experiment) and lipo α‐GC (n = 5, one experiment) mice on Day 7. Data are expressed as means ± SEM. p‐values were determined using an unpaired, two‐tailed Student's t test. *p < .05. (B) Percentages of donor Tregs, and host Tregs among CD4⁺ T cells in spleen cells in vehicle (n = 5, one experiment) and lipo α‐GC (n = 5, one experiment) mice on Day 7. Data are expressed as means ± SEM. p‐values were determined using an unpaired, two‐tailed Student's t test. *p < .05. (C) Percentages of total Tregs among CD4⁺ T cells in spleen cells in syngeneic/vehicle (n = 3, one experiment), syngeneic/lipo α‐GC (n = 3, one experiment), allogeneic/vehicle (n = 5, one experiment) and allogeneic/lipo α‐GC (n = 5, one experiment) mice on Day 7. Data are expressed as means ± SEM. P‐values were determined using an unpaired, two‐tailed Student's t test. *p < .05. BMT, bone marrow transplantation; iNKT, invariant natural killer T; α‐GC, α‐Galactosylceramide