Figure 2.

PAQR3 suppressed ALL cells proliferation. (A) The PAQR3 mRNA expression was measured through RT‐qPCR in MOLT3, MOLT4, CEM‐C1, and Jurkat cells. (B) The PAQR3 protein expression was detected through western blot in MOLT3, MOLT4, CEM‐C1, and Jurkat cells. (C) The overexpression efficiency of PAQR3 was verified through RT‐qPCR CEM‐C1 and Jurkat cells. (D) The cell proliferation was identified through CCK‐8 assay after overexpressing PAQR3. (E) The cell proliferation was also examined through EdU assay after PAQR3 upregulation. **p < .01. ALL, acute lymphoblastic leukemia; CCK‐8, Cell Counting Kit‐8; EdU, 5‐ethynyl‐2‐deoxyuridine; mRNA, messenger RNA; RT‐qPCR, quantitative reverse transcription polymerase chain reaction