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. 2021 May 6;9(3):827–839. doi: 10.1002/iid3.437

Figure 6.

Figure 6

Nrf2 overexpression rescued the effects of PAQR3 on ferroptosis. (A) The cell viability of CEM‐C1 cells was inspected through CCK‐8 assay in the Vector + Erastin, PAQR3 + Erastin, Nrf2 + Erastin, and PAQR3 + Nrf2 + Erastin group. (B–D) The levels of MDA, DCF, and Fe in CEMC1 cells were measured through the appropriate kits in the Vector + Erastin, PAQR3 + Erastin, Nrf2 + Erastin, and PAQR3 + Nrf2 + Erastin group. (E) The apoptosis rate of CEM‐C1 cells was detected through flowcytometry in the Vector + Erastin, PAQR3 + Erastin, Nrf2 + Erastin, and PAQR3 + Nrf2 + Erastin group. (F) The cell viability of CEM‐C1 and Jurkat cells was inspected through CCK‐8 assay in the Vector + RSL3, PAQR3 + RSL3, Nrf2 + RSL3, and PAQR3 + RSL3 + Erastin group. (G–I) The levels of MDA, DCF, and Fe in CEM‐C1 and Jurkat cells were measured through the appropriate kits in the Vector + RSL3, PAQR3 + RSL3, Nrf2 + RSL3, and PAQR3 + RSL3 + Erastin group. (J) The apoptosis rate of CEM‐C1 and Jurkat cells was detected through flowcytometry in the Vector + RSL3, PAQR3 + RSL3, Nrf2 + RSL3, and PAQR3 + RSL3 + Erastin group. *p < .05,**p < .01; # p < .05, ## p < .01. CCK‐8, Cell Counting Kit‐8