Table 1.
List of instrumental and neuronal parameters used in the different model configurations
| Total instrumental capacitance |
Access resistance |
Seal resistance |
Capacitance neutralization |
Bridge balance |
Morphologic representation |
Specific membrane capacitance |
Internal resistivity |
Specific membrane resistivity |
Maximal Na+ conductance |
Maximal K+ conductance |
|
|---|---|---|---|---|---|---|---|---|---|---|---|
| Symbol (unit) | Ctot (pF) | Raccess (MΩ) |
Rseal (GΩ) | CPN (pF) | BB (MΩ) | Cm (μF/cm2) |
Ri (Ω*cm) | Rm (kΩ/cm2) | gmaxNa (mS/cm2) |
gmaxK (mS/cm2) |
|
| AP reconstitution/native AP rediction (Figs. 4-6) |
7.097 | 53.2 | 65.64 | 6.5/7/7.386 | 185 | Detailed anatomic reconstruction |
0.65 | 147.3 | 60.01 | 159.02/151.7/ 160.03*1 |
5.83/5.41/ 6.32*1 |
| Correlations between signal distortions and recording conditions (Fig. 7) |
6.74 | 1–200 | 50/5*2 | 6.8/ 6.3 |
1–200 | Single isopotential compartment (100 cm2/1000 cm2 surface area) |
1 | 1e-9 | 50 | 150 | 40 |
| Correlations between signal distortions and axonal morphology (Fig. 8) |
6.74 | 60 | 50 | 6.8 | 60 | Detailed anatomic reconstruction |
1 | 150 | 50 | 300 | 15 |
| Testing reliability of Ctot estimation (Extended Data Fig. 4-1B) |
5.8–13.7 | n/a.*3 | 50 | n/a.*3 | n/a.*3 | n/a.*3 | n/a.*3 | n/a.*3 | n/a.*3 | n/a.*3 | n/a.*3 |
| Testing reliability of cellular passive parameter and Raccess prediction (Extended Data Fig. 4-1C) |
6.74 | 60 | 50 | 6.8 | 60 | Axon-like realistic morphology (length = 300 μm, mean diameter = 0.6 μm) |
0.5–1.5 | 50–250 | 10–100 | n/a. | n/a. |
| Testing reliability of the prediction of native AP shapes (Extended Data Fig. 4-1D,E) |
6.74 | 100 | 50 | 6.8 | 100 | Single compartment (length, diameter = 15 μm) |
1 | 150 | 10 | 174*4 | 21*4 |
*1mean of the predicted maximal conductance densities for the reconstruction of APs recorded with CPN = 6.5, 7, and 7.386 pF.
*2for the small and for the larger cell, respectively.
*3not applicable in the simulated on-cell VC recording configuration.
*4median of the predicted maximal conductance densities for the reconstruction of APs generated with independent conductance mechanisms.