Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 25;40(31):4955–4966. doi: 10.1038/s41388-021-01904-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A GLI1 expression (top) and representative pERK/ERK and pAKT/AKT western blot analyses (bottom) (n = 2) of KRAS-expressing RUCH-2 cells after siRNA (100 nM each)-mediated ERK1 and/or ERK2 knockdown with and without PI-103 treatment (3 µM) compared to scramble (scr) siRNA transfected KRAS-expressing RUCH-2 and pMSCV control cells. HSC70 served as loading control. B Representative western blot analyses (n = 5) (left) and respective densitometric analyses (right) of GLI1 expression in cytosolic and nuclear fractions of HRAS-, KRAS- and NRAS-expressing RUCH-2 cells with or without SCH772984 treatment in comparison to solvent-treated pMSCV control. Lamin B1 or α-Tubulin served as loading controls for nuclear or cytosolic fractions, respectively. Bars: mean + SEM. * or ^$: significant compared to solvent-treated pMSCV control or solvent-treated oncRAS cell line tested by Mann–Whitney test. *^/$ p < 0.05, **^/$$ p < 0.01, ***^/$$$ p < 0.001, ****^/$$$$ p < 0.0001.