Fig. 5.

Osteoblast behavior on the 2 sizes of BS granules. (a, b) Viability assay of Saos-2 cells cultured on 2 sizes of BS granules on (a) day 1 and (b) day 3. (c–l) Real-time PCR of Saos-2 cells seeded on 2 BS granules was performed to assess the expression of the genes encoding (c, d) TGF-β, (e, f) Runx2, (g, h) COL1, (i, j) ALP, and (k, l) OCN at 3 days (c, e, g, i, and k) and 14 days (d, f, h, j, and l) after cell seeding. (m) Immunochemical staining of OCN on undecalcified frozen sections of Saos-2 cells cultured on BS granules for 14 days (* and light blue-colored areas denote significant differences between the groups, p < 0.05)002E