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. 2021 Aug 5;12(8):770. doi: 10.1038/s41419-021-04045-4

Fig. 4. CDG and CDDG lines exhibit elevated autophagy levels.

Fig. 4

Representative immunofluorescence images of parental RPE-1 cells and CDDG and CDG cell lines stained with antibodies against A p62/SQSTM1 or C LAMP1 in combination with anti-CHOP or anti-PDI antibodies, respectively. Scale bar, 20 µm. Quantification of p62/SQSTM1 (B) and LAMP1 (D) staining by flow cytometry (n ≥ 3 per each genotype or treatment). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA followed by Dunnett multiple comparisons post-test.