Table 5.

Immunological, redox parameters and global methylation DNA in the intestinal wall of turkeys at 9 weeks of age.

	IgY µg/g	IgA µg/g	Casp 8 ng/g	Casp 3 ng/g	IL-6 pg/g	TNF pg/g	Cp U/g	CRP mg/g	OGG1 ng/g	APEX 1 ng/g	3-NT ng/g	8-OHdG ng/g	PC nmol/mg protein	% DNA methylation
Treatment1
LLAL	3250	590.3	44.40	24.26	1469	620.1	0.014	0.029	91.14	5.111	125.57	88.82	0.877	43.95
LLAH	3113	551.1	40.80	22.26	1382	648.7	0.012	0.025	91.63	4.786	112.88	86.03	0.785	47.22
LHAL	3096	552.9	39.70	19.76	1371	667.0	0.012	0.024	84.17	4.851	117.34	83.75	0.356	41.42
LHAH	3322	557.4	39.76	18.27	1379	666.1	0.011	0.020	90.45	4.976	79.89	73.14	0.364	49.26
SEM	71.86	10.77	1.317	0.814	32.86	15.13	0.0004	0.001	1.606	0.093	6.086	1.614	0.053	1.372
Lysine (Lys)
Low (L)	3181	570.7	42.60	23.26a	1426	634.4	0.013	0.027a	91.39	4.948	119.22	87.42a	0.831	45.59
High (H)	3209	555.1	39.73	19.02b	1375	666.5	0.011	0.022b	87.31	4.913	98.61	78.45b	0.360	45.34
Arginine (Arg)
Low (L)	3173	571.6	42.05	22.01	1420	643.5	0.013	0.026	87.66	4.981	121.45a	86.29a	0.617	42.68b
High (H)	3217	554.2	40.28	20.27	1381	657.4	0.012	0.023	91.04	4.881	96.38b	79.58b	0.575	48.24a
P value
Lys	0.854	0.482	0.293	0.008	0.464	0.310	0.122	0.034	0.211	0.856	0.071	0.002	< 0.001	0.925
Arg	0.766	0.434	0.513	0.247	0.559	0.658	0.231	0.143	0.296	0.606	0.030	0.014	0.535	0.045
Lys × Arg	0.227	0.326	0.499	0.864	0.483	0.638	0.727	0.973	0.370	0.247	0.270	0.136	0.463	0.397

¹L_LA_L diet with low Lys and low Arg levels; L_LA_H diet with low Lys and high Arg levels; L_HA_L diet with high Lys and low Arg levels; L_HA_H diet with high Lys and high Arg levels.

IgA: immunoglobulin A; IgY: immunoglobulin Y; Casp 3: caspase 3; Casp 8: caspase 8; IL-6: interleukin 6; TNF-α: tumor necrosis factor alpha; Cp – ceruloplasmin; CRP: C-reactive protein; OGG1: oxoguanine glycosylase; APEX-1: endonuclease 1; 3-NT: 3- nitrotyrosine; 8-OHdG: 8-hydroxydeoxyguanosine; PC: protein carbonyl.

^a,bValues in same column with no common superscripts denote a significant difference (P ≤ 0.05).