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Genes and primers used in the study.
| Gene | Primer | Sequence (5ʹ–3ʹ) | Melting temperature (°C) | Product size (nt) |
|---|---|---|---|---|
| ACTB | Forward | TACCCCATTGAACACGGCAT | 58 | 96 |
| Reverse | CTCCTCAGGGGCTACTCTCA | |||
| VIM | Forward | GGAACAATGATGCCCTGC | 58 | 145 |
| Reverse | GCAAAATTCTCCTCCATTTCAC | |||
| IL-6 | Forward | AAGGCGTGGATAGAGAAG | 58 | 158 |
| Reverse | TGACAGATCGGTAACAGAG | |||
| IgA | Forward | TCCGGGTTCACCTTCAGC | 58 | 163 |
| Reverse | CTGTGCTCTGCCCGTTGT |
ACTB: B-actin; VIM: vimentin; IL-6: interleukin 6; IgA: immunoglobulin A.
