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. 2021 Aug 5;12:4721. doi: 10.1038/s41467-021-25020-9

Fig. 10. Point mutations confirm P6 as an allosteric site.

Fig. 10

The positions of bulky (A, magneta) and tiny (B, purple) mutations around P6. AngII-induced Gq activation (C) and β-arrestin 2 recruitment (D) in HEK293 cells transfected with WT AT1 receptor or mutants. E AngII-induced conformational change of AT1 receptor AT1R conformation sensor in HEK293 cells transfected with AT1R conformation sensor or sensor-based mutants. In (C–E), points for WT, G1.49L, F7.55W, F8.54W, F1.48A, N7.49A, Y7.53A, and F8.50A mutants are colored black, yellow, blue, red, cyan, dark blue, pink, and light yellow, respectively. Three independent experiments were performed, and representative dose–response curves were shown in (C–E). The bars indicate the mean ± SEM values. The data summary was shown in Supplementary Note 22.