Fig. 8. The predicted pockets on the intermediate state and comparison of them with other GPCR allosteric sites.

A All the pockets predicted by Fpocket in the intermediate state. Red circles show the overlap pockets with the prediction of inactive and active representative structures, while blue circles mean the intermediate-only pockets P6 and P9. B The alignment of current allosteric modulators on their sites with intermediate structure and pockets P2, P4, and P8. C The overlap of P9 with current allosteric GPCR modulators in crystal structures. In (B) and (C), cyan cartoons depict the intermediate AT₁ receptor and orange sticks show corresponding pockets defined by Fpocket. Sticks in other colors show the allosteric modulators. AT₁R: angiotensin II type 1 receptor.