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. 2021 Aug 5;11:15911. doi: 10.1038/s41598-021-95146-9

Table 1.

The indicator ASVs characterizing taxa strongly correlated to each biotope, identified using the indicator species analysis procedure implemented in the ‘indicspecies’ package.

ASVsa Indval statistic Phylum Class Order Genus
Adult EAB gut
Asvb11 0.913*** Bacteroidetes Flavobacteria Flavobacteriales Flavobacterium
Asvb24 0.912*** Proteobacteria Gammaproteobacteria Enterobacteriales Serratia
Asvb14 0.882*** Proteobacteria Alphaproteobacteria Caulobacterales Caulobacter
Asvb28 0.881*** Proteobacteria Alphaproteobacteria Rhizobiales Bradyrhizobium
Asvb47 0.878** Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonas
Asvb20 0.849*** Proteobacteria Gammaproteobacteria Alteromonadales Alishewanella
Asvb63 0.816*** Proteobacteria Gammaproteobacteria Xanthomonadales Luteibacter
Asvb31 0.782*** Proteobacteria Gammaproteobacteria Enterobacteriales Unassigned
Asvb73 0.782*** Proteobacteria Gammaproteobacteria Enterobacteriales Unassigned
Asvb25 0.782*** Proteobacteria Alphaproteobacteria Caulobacterales Unassigned
Asvb64 0.745*** Proteobacteria Gammaproteobacteria Enterobacteriales Unassigned
Asvb278 0.707*** Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonas
Ash leaves
Asvb874 0.999*** Bacteroidetes Cytophagia Cytophagales Hymenobacter
Asvf1 0.957*** Ascomycota Dothideomycetes Pleosporales Unassigned
Asvf39 0.942*** Ascomycota Unassigned Unassigned Unassigned
Asvb776 0.913*** Bacteroidetes Cytophagia Cytophagales Hymenobacter
Asvb625 0.913*** Bacteroidetes Cytophagia Cytophagales Hymenobacter
Asvf3 0.913*** Ascomycota Dothideomycetes Pleosporales Unassigned
Asvf11 0.890*** Ascomycota Dothideomycetes Pleosporales Unassigned
Asvf23 0.875*** Ascomycota Dothideomycetes Myriangiales Unassigned
Asvf7 0.862*** Ascomycota Dothideomycetes Pleosporales Pyrenochaeta
Asvf48 0.849*** Ascomycota Dothideomycetes Dothideales Unassigned
Asvb56 0.845*** Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonas
Asvb68 0.843*** Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonas
Asvf89 0.821*** Ascomycota Dothideomycetes Capnodiales Mycosphaerella
Asvf86 0.816*** Ascomycota Dothideomycetes Dothideales Unassigned
Asvf46 0.813*** Ascomycota Dothideomycetes Pleosporales Unassigned
Asvf159 0.782*** Ascomycota Dothideomycetes Pleosporales Unassigned
Asvf215 0.779*** Ascomycota Dothideomycetes Capnodiales Mycosphaerella
Asvf346 0.751*** Ascomycota Dothideomycetes Pleosporales Lewia
Asvb903 0.707*** Proteobacteria Betaproteobacteria Burkholderiales Massilia
Asvf141 0.707*** Ascomycota Unassigned Unassigned Unassigned

*** p ≤ 0.001; ** p ≤ 0.01.

The result was generated by using the multipatt() function of the indicspecies package, based on the species-site group association named Indval.g. The minimal significance alpha = 0.001 and 9999 permutations of samples among the two biotopes. The highest Indval statistic indicate the accuracy of the taxa and the maximum is 1.

aAsvb ASV assigned to bacteria, Asvf ASV related to fungi.