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. 2021 Aug 5;11:15889. doi: 10.1038/s41598-021-95434-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Donor cell integration leads to increased expression of collagen and Matrix metalloproteinases. (A–F) Summary data showing mRNA fold change for ECM-related and matrix metalloproteinase genes: (grey bars): Collagen 1a (Col1a; A), Collagen 4a (Col4a; B) Fibronectin 1 (Fn1; C), Elastin (Eln; D), Laminin a1 (Lama1: E), Laminin b1 (Lamb1: F), Matrix metalloproteinase-2 (Mmp2; G), Matrix metalloproteinase-8 (Mmp8; H), Matrix metalloproteinase-9 (Mmp9; I) and Matrix metalloproteinase-13 (Mmp13; J) 21-days after ex vivo transplantation compared to non-transplanted control (white bars) tissues. Error bars represent mean ± s.e.m. n = 4 for each gene examined. *P ≤ 0.05, **P ≤ 0.01 comparing ΔCT values by Welch’s t-test.