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. 2021 Aug 5;11:15892. doi: 10.1038/s41598-021-95158-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of Alpha-2 adrenergic receptor in colon. (A) The excised sections were showed by zones. (B, C) The mean grayscale value (MGV) of Alpha-2 adrenergic receptors in colonic smooth muscle layer (B) and colonic mucosa layer (C) 12 weeks after PSIMA. *SCI rats with S versus SCI rats, ^†Rats with S versus Intact rats in Zone 3, 4, 5, P < 0⋅05 (two-way ANOVA). (D) Colonic tissue (TRI staining), the smooth muscle tissues are directed by the yellow arrows, and the colonic mucosa tissue by the blue arrows. (E, F) Representative fluorescent images of Alpha-2 adrenergic receptors (red) in colonic tissue, cross-sectional anatomy of colon (E), and high-power images (F).