Table 1.

Anaplasma marginale, A. ovis, A. phagocytophilum, and GADPH gene (internal control) RPA primers and probes*.

Target Seq	Primer	Length	Tm	GC%	Product size (bp)	Sequence
A. marginale	Am3L_msp4	33	76.63	54.55	103	ACGAAGTGGCTTCTGAAGGGGGAGTAATGGGAG
	Am3R_msp4	30	72.99	50		[5′Label-Biotin] GACTCACGCATGTCGAACGAGGTAACAGAA
	Am3_probe	39	70.4	56.4		[5′-Label-FAM] TAGCTTTTACGTGGGTGCGGCCT[THF]CAGCCCAGCATTTCC[3′-block-C3spacer]
A. phagocytophilum	ApL_msp4	30	75.14	60	202	TGCGGCCGCAGTATGTGCCTGCTCCCTTTT
	ApR_msp4	30	62.31	40		[5′Label-Biotin] GTTATAACCTTTTACGTAAGATCCTCCCCT
	Ap_probe	52	68.2	44.2		[5′Label-FAM] TGATGCGTCTGATGTTAGCGGTGTTATGAACGG[THF]AGCTTTTACGTAAGTGGT [3′block-C3spacer]
A. ovis	Ao2L_msp4	35	67.75	45.71	184	AGAGACCTCGTATGTTAGAGGCTATGACAAGAGTG
	Ao2R_msp4	35	68.57	48.57		[5′Label-Biotin] CCTTCTGTAGCTTGCTTCTAGTTCCACTCTAGCTC
	Ao2_probe	50	65.2	38		[5′Label-FAM] AAATCCGGCTACACTTTTGCTTTCTCTAAGAA[THF]TTACTCACATCTTTCGA [3′block-C3spacer]
GAPDH gene	GAPDH2_L	30	72.53	50	168	CTGAGACAAGATGGTGAAGGTCGGAGTGAA
	GAPDH2_R	27	72.82	51.85		[5′Label-Biotin] TTGCCGTGGGTGGAATCATACTGGAAC
	GAPDH_Probe	51	70.3	51		[5′Label-DIG] CTGGCAAAGTGGACATCGTCGCCATCAATGACCC[THF]TTCATTGACCTTCACT[3′block-C3spacer]

*Primers, probes, and the amplification kit are protected by a patent application.