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. 2021 Aug 5;12:4730. doi: 10.1038/s41467-021-24775-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic illustration of the cerebral organoid culture system with a combination of 3D BEM hydrogel culture and the microfluidic device. b Schematic of the culture protocol for generating cerebral organoids from human induced pluripotent stem cells (iPSCs) using a brain-mimetic culture system. The developmental stage of organoids is shown at the bottom, the timeline for each developmental stage is shown in the middle, and the culture condition is shown at the top. c–j Proteomic analysis for the identification of extracellular matrix (ECM) components in human BEM. The percentages of (c) matrisome proteins out of total proteins, and (d) the subtypes of matrisome proteins identified in BEM, Matrigel (Mat), and human brain tissue (n = 1 for Mat, n = 3 for BEM). e Total numbers of matrisome proteins detected in the human brain tissue and BEM. f The number of the brain-enriched proteins by at least 4-fold compared to other organs. g The top 10 biological process terms ordered by p value after gene ontology enrichment analysis of brain-enriched proteins exclusively present in BEM, which are not detected in Mat. The compositions of (h) matrisome proteins relative to total proteins and (i) the subtypes of matrisome proteins in three batches of BEM (biological replicates = 3). j Most abundant matrisome proteins found in each batch of BEM. Source data are provided as a Source Data file.