Table 1.

Computational analyses of the seven human ERdJ proteins.

Length, size	Signal peptide probability	Cleavage probability	J-domain	TM domain	Other domains
Name: ERdj1, synonymous: ER1p, MTj1, HTJ126
554 aa 63.8 kDa	79%	aa 47/48 97.4%	aa 65–126	N-terminus inside aa 30 … 48 i – o aa 154 …171 o –i aa 222 …242 i – o alternative model aa 30 … 48 o – i aa 149 …170 i – o	Myb DNA binding domain aa 329…374 aa 495…540
Name: ERdj2, synonymous: DNAJC23, PCLD2, PRO2507, SEC63L
760 aa 87.8 kDa	None 0.2%		aa 104–161	N-terminus inside aa 15 … 35 o – i aa 76 … 92 i – o aa 193 …212 o – i alternative model aa 15 … 34 i – o aa 73 … 91 o – i aa 190 … 208 i - o
Name: ERdj3, synonymous: DNAJB11, apobec-1-binding protein-2, PWP1-interacting protein 4, apobec-1 binding protein, HEDJ
358 aa 40.5 kDa	84%	aa 22/23 53.2%	aa 25–87	N-terminus outside aa 6… 23 o – i aa 105…121 i – o alternative model aa 1… 23 i - o
Name: ERdj4, synonymous: Mdg1, Mdj7, DnaJB9
222 aa 25.7 kDa	80.4%	aa 23/24 66%	aa 26–87	N-terminus inside aa 2…22 i – o alternative model aa 2 … 22 o - i	U4/U6.U5 small nuclear ribonucleoproteins: aa 170–198
Name: ERdj5, synonymous: DnajC10, JPDI
793 aa 91.1 kDa	None 3.3%		aa 35–97	N-terminus inside aa 17… 37 i – o aa 245 … 264 o –I alternative model aa 16 … 32 o – i aa 245 … 264 i – o	Thioredoxin domains 131 … 229 466 … 558 … 641 674 … 762
Name: ERdj6, synonymous: DnajC3, p58IPK
504 aa 57.6 kDa	62.3%	aa 31/32 32%	aa 394–459	N-terminus outside aa 3 … 26 o – i alternative model aa 7 … 26 i - o	Multiple tetratricopeptide repeats (TPRs)
Name: ERdj7
295 aa 34.7 kDa	99.7%	aa 22/23 49.7%	aa 29–90	N-terminus outside aa 1 … 17 o – i aa 119 … 137 i – o aa 202 … 223 o – i alternative model aa 2 … 19 i – o aa 119 … 135 o – i aa 208 … 227 i – o

Programs used for theoretical analyses: Calculated mass: https://web.expasy.org/cgi-bin/compute_pi/pi_tool; Potential motifs and transmembrane structure https://www.genome.jp/tools/motif/.

Potential signal sequences and their cleavage probability http://www.cbs.dtu.dk/services/SignalP/.