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. 2021 Aug 5;12:4734. doi: 10.1038/s41467-021-25057-w

Fig. 1. Melanoma growth after intratumoral artLCMV-TRP2 treatment.

Fig. 1

a Schematic depiction of the recombinant artLCMV vector expressing the melanocyte differentiation antigen TRP2 (artLCMV-TRP2) and treatment scheme. b Tumor growth with indication of number of tumor-free mice and c survival of mice treated intratumorally (i.t.) or intravenously (i.v.) with artLCMV-TRP2. d Tumor kinetics of B16F10 and TRP2-deficient B16F10 (B16F10-Dct−/−) in mice immunized i.t. with artLCMV-TRP2. e Tumor growth with indication of number of tumor-free mice of mice treated i.t. with artLCMV-TRP2 or -GFP. f Tumor growth in mice administered with anti-CD8- and anti-CD4-depleting antibodies and treated i.t. with artLCMV-TRP2. g Surviving mice from c were re-challenged with B16F10 s.c. into the opposite right flank. In one cohort of mice, CD8- and CD4-depleting antibodies were administered. h, i Mice were inoculated with s.c. B16F10 on day 0 and i.v. on day 3 to establish pulmonary melanoma metastasis. On day 7, mice were treated i.t. with artLCMV-TRP2 and on day 17 lungs were harvested. Analysis of lungs for pulmonary tumor metastasis with representative images (h) and quantification (i). Dots indicate mean ± s.e.m. values for each time point. Pooled data from three independent experiments with n = 14 (PBS), n = 13 (artLCMV-TRP2, i.v.) and n = 15 (artLCMV-TRP2, i.t.) mice (b, c), n = 15 (Untreated), n = 13 (Survivors) and n = 7 (Survivors + α-CD8/α-CD4) mice (g); n = 10 (PBS, i.t.) and n = 11 (artLCMV-TRP2, i.t.) mice (i). Pooled data from two independent experiments with n = 9 (B16F10, PBS), n = 6 (B16F10-Dct−/−, PBS), n = 14 (B16F10, artLCMV-TRP2, i.t.) and n = 7 (B16F10-Dct−/−, artLCMV-TRP2, i.t.) mice (d); n = 10 (PBS), n = 9 (artLCMV-GFP, i.t.) and n = 9 (artLCMV-TRP2, i.t.) mice (e); n = 9 (PBS), n = 11 (artLCMV-TRP2, i.t.) and n = 10 (artLCMV-TRP2, i.t.+α-CD8/α-CD4) mice (f). Statistical analysis was performed using two-way analysis of variance (ANOVA) with Bonferroni (b, d, e, f), Log-Rank Mantel–Cox test (c, g), or unpaired two-tailed Student’s t test (i) with *P < 0.05: **P < 0.01; ***P < 0.001. Source data and exact P values are provided in the Source data file.