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. 2021 Aug 5;12:4734. doi: 10.1038/s41467-021-25057-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Mice were inoculated s.c. with B16F10 melanoma cells and treated on day 7 i.t. with artLCMV-TRP2. Frequency of ST2⁺ CD8⁺ T cells in the tumor and the TDLN at indicated time points. b Tumor growth kinetics and c survival in Cxcl13-Cre Il33^fl/fl and Cre-negative littermate mice (Ctrl) treated i.t. with artLCMV-TRP2 on day 7 after B16F10 inoculation. Dots indicate mean ± s.e.m. for each time point. d–k Tumor-infiltrating T cells were analyzed by flow cytometry on day 15. d Frequency of tumor-infiltrating CD8⁺ and CD4⁺ T cells. e Frequency of TRP2 tetramer⁺ CD8⁺ T cells and f KLRG1⁺ CD62L⁻ CD8⁺ T cells. g IFN-γ- and TNF-α-producing tumor-infiltrating TRP2-specific CD8⁺ T cells. h Frequency of CD69⁺ CD8⁺ T cells. i Mean expression of Eomes, PD-1, TOX, and T-bet on tumor-infiltrating CD8⁺ T cells and j Frequency of PD-1^hi Eomes^hi and k PD-1^hi TOX⁺ among CD8⁺ T cells. Dots represent individual mice and lines indicate mean values. Pooled data from two independent experiments with n = 8 (Day 11), n = 10 (Day 13) and n = 9 (Day 15) (a); n = 11 (PBS, Ctrl), n = 8 (PBS, Cxcl13-Cre Il33^fl/fl), n = 9 (artLCMV-TRP2, i.t., Ctrl) and n = 10 (artLCMV-TRP2, i.t., Cxcl13-Cre Il33^fl/fl) mice (b, c); n = 8 (Ctrl) and n = 10 (Cxcl13-Cre Il33^fl/fl) mice (f); n = 10 (Ctrl) and n = 8 (Cxcl13-Cre Il33^fl/fl) mice (g); n = 5 (Ctrl) and n = 10 (Cxcl13-Cre Il33^fl/fl) mice (h); n = 7 (TOX, Ctrl) and n = 9 (TOX, Cxcl13-Cre Il33^fl/fl) mice (i); n = 7 (Ctrl) and n = 11 (Cxcl13-Cre Il33^fl/fl) mice (k). Pooled data from three independent experiments with n = 10 (Ctrl) and n = 15 (Cxcl13-Cre Il33^fl/fl) mice (d, e, j); n = 7 (PD-1, Ctrl), n = 9 (T-bet, Ctrl), n = 10 (Eomes, Ctrl) and n = 9 (PD-1, Eomes, T-bet, Cxcl13-Cre Il33^fl/fl) mice (i). Statistical analysis was conducted using one-way ANOVA with Tukey’s multiple comparison test (a), two-way analysis of variance (ANOVA) with Bonferroni (b), Log-Rank Mantel–Cox test (c) or unpaired two-tailed Student’s t test (d–k) with *P < 0.05: **P < 0.01; ***P < 0.001. Source data and exact P values are provided in the Source data file.