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. 2021 Aug 5;12(8):772. doi: 10.1038/s41419-021-04042-7

Fig. 6. Gene manipulation of STAT3 affects the enhanced antitumor effect of APG-1252 in combination with gemcitabine.

Fig. 6

a, b The apoptotic rate of the indicated CNE2, HNE1 cells transfected with the STAT3 shRNA, scrambled control, and STAT3 overexpression vectors after treatment with or without the combination therapy were determined using an Annexin V/PI assay. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the standard deviation of three independently performed experiments. c, d Typical images and enumeration of the indicated cells (scramble control, STAT3 shRNA, vector, STAT3 overexpression) treated with or without the combination therapy after crystal violet staining. Data are shown as the mean±SD. *P < 0.05, **P < 0.01, ***P < 0.001. e, f the CCK-8/IC50 values of the indicated CNE2 and HNE1 cells transfection with the STAT3 shRNA, scrambled control, and STAT3 overexpression vectors after treatment with the combination therapy. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the standard deviation of three independently performed experiments. SC Scramble control, PI propidium iodide, STAT3 signal transducer and activator of transcription 3, shRNA short hairpin RNA, CCK-8 Cell Counting Kit-8.