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. 2021 Jul 13;297(2):100960. doi: 10.1016/j.jbc.2021.100960

Figure 1.

Figure 1

Northern blot analysis of 22 mt-tRNAsteady-statelevels among five mouse tissues.A and C, four micrograms of total RNA from mouse-derived brain, heart, liver, skeletal muscle, and kidney tissues was electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with DIG-labeled oligonucleotide probes specific for the 22 mt-tRNAs, ct-tRNAAsp(GTC), tRNALys(TTT) and tRNALys(CTT) and 5S rRNA, respectively. B and D, quantification of relative mt-tRNA levels of the five tissues. The content of each mt-tRNA was normalized to that of 5S rRNA in the five tissues. Relative tRNA levels for the brain, heart, liver, skeletal muscle, and kidney were normalized to the mean values of each tRNA among the five tissues. Calculations were based on 3 to 5 independent experiments. Error bars indicate two standard deviations (SD) of the means. B, Brain; H, Heart; K, Kidney; L, liver; M, skeletal Muscle.