Figure 2.

APM gel electrophoresis combined with Northern blot analysis of mt-tRNAs.A, four, 2.3, 7, 3.5, and 3.4 μg of total RNAs from the brain, heart, liver, skeletal muscle, and kidney tissues were separated by polyacrylamide gel electrophoresis containing 0.05 mg/ml APM, electroblotted onto a positively charged membrane, and hybridized with a DIG-labeled oligonucleotide probe specific for mt-tRNA^Gln, tRNA^Glu, tRNA^Lys, and tRNA^Leu(UUR), respectively. B, quantification of 2-thiolated tRNA levels. Calculations were based on more than three independent experiments. Error bars indicate SD of the means.