Figure 5.

Western blotting analysis of mitochondrial proteins.A, eight micrograms of total cellular proteins from five mouse-derived tissue types was electrophoresed through a denaturing polyacrylamide gel, electroblotted, and corresponding Coomassie Brilliant Blue stained gel were used as loading control. The blots were hybridized with antibodies for 11 subunits of OXPHOS (six encoded by mtDNA and seven encoded by nDNA). B, quantification of OXPHOS subunits levels in the five tissues. The content of each proteins was normalized to that of total cellular protein in the five tissues. Relative protein levels for the brain, heart, liver, skeletal muscle, and kidney tissues were normalized to the mean values of each protein among the five tissues. Calculations were based on three independent determinations. Error bars indicate SD of the means.