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. 2021 Jul 10;297(2):100946. doi: 10.1016/j.jbc.2021.100946

Figure 2.

Figure 2

LPS treatment impairs PKA and PKG activities in vivo via peroxynitrite-dependent mechanism. The activity of PKA and PKG was tested in lung extracts obtained from mice treated with LPS and/or MnTMPyP as described in the Experimental procedures section. The protein levels of respective enzymes were evaluated by immunoblotting. LPS treatment did not affect the protein levels of either PKG-Iα (A) or PKA (D). However, LPS attenuated both PKG-Iα (B and C) and PKA (E) activity. Again, MnTMPyP pretreatment preserved the activity of PKA and PKG (B, C, and E). Data are mean ± SEM, n = 3 to 5. ∗p < 0.05 versus control lungs; †p < 0.05 versus LPS alone. LPS, lipopolysaccharide; MnTMPyP, manganese (III) tetrakis(1-methyl-4-pyridyl)porphyrin; PKG, protein kinase G.