Figure 13.

Foz+WD mice progress from NASH to cirrhosis and HCC by 24 weeks. (A) Representative gross liver images from 24-week WD-fed mice showing macroscopic tumor nodules in Foz+WD livers (white circles). (B) Tumor incidence and (C) visible tumor numbers (>1 mm) were plotted. Representative H&E-stained tissue sections showing (D) trabecular and (E) steatohepatitic HCC in Foz+WD 24-week livers. Classic HCC markers AFP, YAP1, and GPC3 were detected either by (F) IHC or (G) qRT-PCR. RNA from Foz+chow at 24 weeks was used as an age matched control for the gene expression. Data are expressed as the means ± SEM. (C) One-way analysis of variance and (G) unpaired t test. ∗P < .05, ∗∗P < .01. Scale bar: 200 μm, or as indicated. NT, nontumor region from tumor-bearing livers; Tu, tumor tissue.