Table 3.
List of Antibodies for Immunohistochemistry, Immunoblot, and FACS
| Antibody | Catalog | Vendor | Fluorochrome |
|---|---|---|---|
| Immunohistochemistry | |||
| Desmin | RB-9014-P0 | Thermo Fisher | NA |
| αSMA | ab5694 | Abcam | NA |
| F4/80 | 14-4801-88 | eBioscience, San Diego, CA | NA |
| GPC3 | ab66596 | Abcam | NA |
| AFP | CP028A | Biocare Medical, Pacheco, CA | NA |
| YAP | CS12640 | Cell Signaling | NA |
| Immunoblot | |||
| αSMA | ab5694 | Abcam | NA |
| Desmin | RB-9014-P0 | Thermo Fisher | NA |
| P62 | CS23214 | Cell Signaling | NA |
| LC3 I/II | L7543 | Millipore-Sigma | NA |
| Chop | CS5554 | Cell Signaling | NA |
| ATF6 | alx804381 | Enzo Life Sciences, Farmingdale, NY | NA |
| Tubulin | T5168 | Millipore-Sigma | NA |
| FACS | |||
| ILC/Treg panel | |||
| Marker antibody | |||
| CD45 | 748370 | BD Biosciences, Franklin Lakes, NJ | BUV805 |
| Lineage plus FCeR1a antibody | 133302 | Biolegend, San Diego, CA | FITC |
| CD90 | 564365 | BD Biosciences | BV 785 |
| ST2 | 746115 | BD Biosciences | BB700 |
| GATA3 | 12-9966-42 | Thermo Fisher | PE |
| RORyt | 17-6988-82 | Thermo Fisher | APC |
| CD127 | 135041 | Biolegend | BV605 |
| Tbet | 644816 | Biolegend | BV421 |
| NK1.1 | 47-5941-82 | Thermo Fisher | APCFluor 780 |
| TCRab | 748406 | BD Biosciences | BUV563 |
| Foxp3 | 25-5773-82 | Thermo Fisher | Pecy7 |
| Live dead | 423108 | Biolegend | BUV496 |
| CD25 | 564022 | BD Biosciences | BUV 395 |
| CD4 | 612843 | BD Biosciences | BUV 737 |
| Myeloid cells panel | |||
| CD11b | 612800 | BD Biosciences | BUV 737 |
| CD3 dump channel | 563565 | BD Biosciences | BUV 395 |
| B220 dump channel | 563793 | BD Biosciences | BUV 395 |
| TER119 dump channel | 563827 | BD Biosciences | BUV 395 |
| CD45 | 748370 | BD Biosciences | BUV 805 |
| F4/80 | 749284 | BD Biosciences | BUV 563 |
| CD11c | 563735 | BD Biosciences | BV786 |
| CD206 | 141721 | Biolegend | BV605 |
| MerTK | 47-5751-82 | Thermo Fisher | APCFluor 780 |
| Trem2 | MA5-28223 | Thermo Fisher | FITC |
| IRF5 | 158603 | Biolegend | PE |
| CD80 | 104726 | Biolegend | 421 |
| MHC-II | 25-5321-82 | Thermo Fisher | APC |
| Ly6C | 45-5932-82 | Thermo Fisher | PeCy7 |
| Ly6G | 25-9668-82 | Thermo Fisher | BV480 |
| Live dead | 423108 | Biolegend | BUV496 |
| Siglec F | 740764 | BD Biosciences | BUV 711 |
αSMA, α-smooth muscle actin; MHC-II, major histocompatibility complex class II; TCRαβ, T-cell receptor α/β; Treg, regulatory T cell.