Fig. 3.
TRIM31 is indispensable for anti-fungal Th1 and Th17 responses. a qRT-PCR analysis of Ifng, Il17a, and Il17f from kidneys of Trim31+/+ (n = 6) and Trim31−/− (n = 6) mice infected with C. albicans (2 × 105 fungal cells per mouse) for 5 days. b ELISA analysis of IFN-γ (left) and IL-17A (right) in the supernatant of splenic cells obtained from Trim31+/+ and Trim31−/− mice (n = 6 per group) infected with C. albicans (2 × 105 fungal cells per mouse) for 5 days, followed by stimulation with HKCA-Y (MOI, 1) for 2 days. c, d Splenic cells isolated from the Trim31+/+ or Trim31−/− mice and stimulated as in (b). Intracellular staining of IFN-γ (Th1) and IL-17A (Th17) were determined with flow cytometry. The representative figure is shown in (c), and the results are summarized in (d). e ELISA analysis of IgG in serum from Trim31+/+ and Trim31−/− mice (n = 6 per group) infected with C. albicans (2 × 105 fungal cells per mouse) for 5 days. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001 (Student’s t-test in a, b, d, e). Data are from one experiment representative of three independent experiments (a, b, d, e; mean ± s.d.)