TABLE 2.
Amplitude of ECG components on Einthoven leads in zebrafish vs. humans.
| Amplitude (mV) | P wave Mean ± SD | QRS complex Mean ± SD | T wave Mean ± SD | P:QRS:T |
| Zebrafish lead I | ||||
| Total (n = 30) | 0.028 ± 0.049 | 0.126 ± 0.049 | 0.006 ± 0.006 | 0.36:1.00:0.15 |
| M (n = 15) | 0.016 ± 0.011 | 0.080 ± 0.011 | 0.006 ± 0.007 | 0.29:1.00:0.11 |
| F (n = 15) | 0.041 ± 0.067 | 0.172 ± 0.178 | 0.008 ± 0.007 | 0.42:1.00:0.19 |
| Zebrafish M vs. F lead I P-value [95% CI] | 0.4 [−0.007,0.025] | 0.3 [0.017,0.128] | 0.3, [−0.002,0.005] | |
| Zebrafish lead II | ||||
| Total (n = 30) | 0.037 ± 0.032 | 0.081 ± 0.059 | 0.004 ± 0.003 | 0.55:1.00:0.08 |
| M (n = 15) | 0.046 ± 0.039 | 0.098 ± 0.072 | 0.005 ± 0.003 | 0.57:1.00:0.09 |
| F (n = 15) | 0.028 ± 0.020 | 0.063 ± 0.037 | 0.004 ± 0.002 | 0.52:1.00:0.08 |
| Zebrafish M vs. F lead II P-value [95%CI] | 0.2 [−0.034,0.006] | 0.1 [−0.054,0.008] | 0.5 [−0.004,0.001] | |
| Zebrafish lead I vs. II P-value [95% CI] | 0.008 [0.002,0.020] | 0.6 [−0.100,0.015] | 0.08 [−0.004,0.001] | |
| Human lead I | ||||
| Total (n = 24) | 0.08 ± 0.03 | 0.49 ± 0.35 | 0.25 ± 0.09 | 0.16:1.00:0.51 |
| M (n = 12) | 0.09 ± 0.02 | 0.47 ± 0.34 | 0.28 ± 0.09 | 0.19:1.00:0.60 |
| F (n = 12) | 0.08 ± 0.03 | 0.51 ± 0.37 | 0.22 ± 0.07 | 0.16:1.00:0.43 |
| Human M vs. F lead I P-value [95% CI] | 0.2 [−0.05,0.00] | 0.8 [−0.3,0.4] | 0.08 [−0.11,0.00] | |
| Human lead II | ||||
| Total (n = 24) | 0.11 ± 0.05 | 0.93 ± 0.28 | 0.33 ± 0.11 | 0.12:1.00:0.35 |
| M (n = 12) | 0.12 ± 0.05 | 0.90 ± 0.34 | 0.38 ± 0.11 | 0.13:1.00:0.42 |
| F (n = 12) | 0.10 ± 0.03 | 0.95 ± 0.23 | 0.28 ± 0.10 | 0.11:1.00:0.29 |
| Human M vs. F lead II P-value [95% CI] | 0.3 [−0.05,0.00] | 0.6 [−0.18,0.29] | 0.1 [−0.2,0.0] | |
| Human lead I vs. II P-value [95% CI] | 0.004 [0.006,0.050] | <0.0001 [0.12,0.80] | 0.0009 [0.03,0.12] | |
| Fish vs. human lead I P-value [95% CI] | <0.0001 [0.05,0.08] | <0.0001 [0.35,0.64] | <0.0001 [0.21,0.29] | |
| Fish vs. human lead II P-value [95% CI] | <0.0001 [0.06,0.09] | <0.0001 [0.78, 0.91] | <0.0001 [0.296,0.300] |
We compared lead differences (for the same subject) using the Wilcoxon paired signed rank test, sex differences (for the same species and lead) and species differences (for the same lead) using the Mann-Whitney-Wilcoxon test. We estimated P-value and 95% confidence interval [95% CI] by bootstrapping with 10,000 replications. P-values are shown in italics; statistically significant P-values and [95%CI] in bold font.