FIGURE 1.

Drop assays (A) and survival percentage (B) of the nine complete clones and individual predicted ORFs involved in perchlorate-resistance. Cultures were grown overnight at 37°C, drops of 10-fold serial dilutions were plated on LB-Ap containing 125 mM of sodium perchlorate and plates were incubated for 48 h at 37°C. Survival percentage was calculated as the number of CFU mL^–1 remaining after the treatment divided by the initial CFU mL^–1. E. coli DH10B bearing empty pSKII+ (pBlueScript II SK +) vector was used as a negative control. One-way ANOVA followed by Dunnet post hoc analysis revealed the displayed significant differences against the negative control (***p < 0.001). Data represent the mean ± S.D (n ≥ 3).