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Drop assays in the presence of perchlorate of clones overexpressing: (A) pJR12-orf2, E. coli DH10B queF and B. subtilis PY79 queF, and (B) pJR76, pJR76-orf2 and E. coli DH10B yrbC. Cultures were grown overnight at 37°C, drops of 2-fold (A) or 10-fold (B) serial dilutions were plated on LB-Ap containing 125 mM of sodium perchlorate and plates were incubated for 48 h at 37°C. E. coli DH10B bearing empty pSKII+ (pBlueScript II SK +) vector was used as a negative control.
