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4-NQO (4-nitroquinoline-N-oxide) resistance test by drop assays of the individual predicted ORFs involved in perchlorate resistance and their complete clones. Cultures were grown overnight at 37°C, drops of 4-fold serial dilutions were plated on LB-Ap supplemented with 0.2 μM 4-NQO and plates were incubated for 48 h at 37°C.
