Figure 2.

Excretory-secretory products from the fourth stage larva of A. cantonensis could be further classified into AC L4 exosome and AC L4 exofree. (A) Scheme of AC L4 exosomes and AC L4 exofree isolation by differential ultracentrifugation from conditioned medium of the fourth stage larva of A. cantonensis. (B) Morphological characterization of AC L4 exosomes were identified using transmission electron microscope. (C) Concentration and size distribution of AC L4 exosomes were measured by IZON qNano particle analyzer. (D) BMDMs were incubated with PBS (a) or 5 μg/ml PKH26-labeled AC L4 exosomes (b) for 1 h, and a confocal analysis was performed to evaluate exosome internalization. Phalloidin was used to label actin (green), and DAPI was used for the staining of nuclei (blue). Scale bar=20 μm.