Figure 6.

EBs produced by ddbAts mutant cells have a defect in maintenance of EB infectivity at the non-permissive temperature. (A) The ability of EBs to maintain infectivity after formation was determined by treating infected cells grown at 37°C with penicillin at 24 hpi and then shifting the temperature to 40°C. The premade EBs were harvested at 48 hours and maintenance of infectivity measured by IFU assay at 37°C. (B) Maintenance of infectivity was also measured using an axenic growth media. EBs from G5 or the ddbAts mutant were incubated in CIP-1 axenic growth media at 37°C or 40°C for 24 hours before measuring infectivity by an IFU assay. *p < 0.01.