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. 2021 Jul 23;15:714780. doi: 10.3389/fncir.2021.714780

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Copyright © 2021 Bertero, Garcia and Apicella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Auditory cortex injection of AAV-flex-ChRimson-tdTomato. (A) Schematic representation of right auditory cortex viral injection. (B) Representative coronal section of an acute slice of the injection site (200 μm thick), with bright field (gray) and tdTomato expressing neurons (red). The Allen brain atlas coronal table superimposed for reference indicates the correct targeting of the Auditory cortex. Scale bar: 1 mm. (C) Top panel: antero-posterior and dorsal ventral spread of ChRimson-tdTomato injection in AC. Scale Bars: 1 mm. Bottom panel: high magnification image of the injection site showing no evidence of ChRimson-tdTomato deposit or spillover in the hippocampus and/or other subcortical structures. Scale Bars: 500 μm. (D) High magnification confocal image of the injection site showing tdTomato expressing neurons. Scale bar: 100 μm. (E) Top panel: bright field and epifluorescence image of tdTomato expressing VIP neuron used in whole-cell patch clamp recordings. Bottom panel: example of responses recorded from a VIP neuron using red LED at two different powers. (F) Reconstruction of the dendritic arborization of biocytin-filled tdTomato expressing VIP neurons. Scale bar: 100 μm. RSP, retrosplenial cortex; VIS, visual cortex; TeA, temporal association cortex; AC, auditory cortex; HPF, hippocampal formation; CA1, Cornu Ammonis of the hippocampus subfield 1; CA3, Cornu Ammonis of the hippocampus subfield 3; DG, dentate gyrus; TH, thalamus; MGB, medial geniculate body; LGN, lateral geniculate nucleus; MB, midbrain; SN, substantia nigra; HY, hypothalamus.