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. 2021 Jul 23;11:698662. doi: 10.3389/fcimb.2021.698662

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Copyright © 2021 Rollin-Pinheiro, Almeida, Rochetti, Xisto, Borba-Santos, Rozental and Barreto-Bergter

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of miltefosine on oxidative stress (A) and the mitochondrial membrane potential (B) in Scedosporium aurantiacum after 6 h of incubation. DCFH-DA and JC-1 fluorescent staining was used to measure reactive oxygen species (ROS) production and membrane polarisation, respectively. Control (-) represents cells in the absence of fluorescent stain. Control (+) represents cells stained with fluorescent DCFDA or JC-1, but without miltefosine treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.