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In vitro functional characterization of the conjugate. (A) Coagulation inducing activity of tTF and tTF-AS1411. (B) Targeting assessment of the Cy5.5-labeled tTF-AS1411 or various controls to V-HUVECs by flow cytometry analysis. (C) Representative confocal images of V-HUVECs after incubating with Cy5.5-labeled tTF-AS1411 (10 nmol/L) or controls for 30 min. Cell membranes and nuclei were stained with DiO (green) and Hoechst 33342 (blue), respectively. Scale bar = 20 μm.
