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. 2021 Jan 23;11(7):1835–1852. doi: 10.1016/j.apsb.2021.01.015

Figure 5.

Fig. 5

CP-25 inhibits PGE2-induced MH7A proliferation by down-regulating GRK2 translocation. (A) CP-25 inhibited PGE2-induced MH7A proliferation (n = 3). (B) PGE2 induced EP4 receptor short-term desensitization and GRK2 membrane translocation. The membrane expression of EP4 and GRK2 in MH7A treated by PGE2 were detected by Western blot (n = 3). (C) The effect of CP-25 on the expression of GRK2 and EP4 on membrane of MH7A stimulated by PGE2 (n = 3). (D) PGE2 stimulated MH7A lysates were used anti-EP4 antibody by co-immunoprecipitate and subjected to Western blot to visualize GRK2 to evaluate the association with EP4 (n = 3). (E) and (F) The co-expression of GRK2 and EP4 was detected by laser confocal microscopy (n = 6). Scale bars = 50 μm. Data are expressed as mean ± SD. #P < 0.05 vs. control group; ∗P < 0.05, ∗∗P < 0.01 vs. PGE2.