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. 2021 Jul 23;12:659012. doi: 10.3389/fgene.2021.659012

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Copyright © 2021 Parks, Rogers, Prins, Williams, Chen, Jones, Moore and Mulligan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental Overview. (A) Overview of the generation of the BXD RI population. This family of strains has been derived at several points in time from both F2 and advanced intercrosses and there are now ∼150 strains available. (B) Description of daily treatment regime. Initial sensitivity is measured by subtracting Day 1 trait values from those of Day 0 for each individual. (C) Timing of daily trait measurements for body temperature (Temp), time mobile in the open field (OF), and latency to withdrawal the tail in response to a thermal stimulus (TF) are shown as minutes post-injection.