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. 2021 Jul 23;12:686545. doi: 10.3389/fpls.2021.686545

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Copyright © 2021 Feng, Fu, Han, Xu, Liu, Bi and Ai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interactive effects of MT and SNP on P_n, A_sat, activity of Ribulose-1,5-bisphosphate carboxylase (RuBPCase) and Rubisco activase (RCA), and relative expression of rbcL and RCA in mRNA and the protein level in cucumber seedlings under cold stress. (A) P_n, (B) A_sat; (C,D) activity of RuBPCase and RCA, respectively; (E,F) Relative mRNA expression of rbcL and RCA, respectively; and (G,H) Protein level of rbcL and RCA, respectively. Coomassie Brilliant Blue staining Rubisco (RBC) protein is shown for equal loading control. Western blotting was performed three times with three independent biological samples, and similar results were obtained. Three-leaf stage seedlings were foliar sprayed with 100 μM MT, 75 μM SNP, 100 μM cPTIO+100 μM MT, 50 μM p-CPA+75 μM SNP, or deionized water (control) for 24 h and then were exposed to 5°C for 24 h. Data are represented as mean ± SD (n = 3). Different letters indicate a significant difference between samples according to Duncan's new multiple range test (p < 0.05).